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Therefore, hyperhomocysteinemia directly damages neuronal sheath or indirectly through the induction of abnormal immune response (Mititelu et al

Additional primary antibodies used were anti--synuclein (AS) (1:1000, Chemicon or 1:50, Abcam) and anti-tyrosine hydroxylase (TH, 1:8000, Sigma)

The peptide also promotes polarization of macrophages from pro-inflammatory M1 phenotypes toward tissue-repairing M2 phenotypes, with treated macrophages showing 70% increased phagocytic activity for clearing inflammatory debris and 3-fold higher production of VEGF compared to untreated controls

For researchers studying aging mechanisms, this convergence provides a molecularly coherent framework for understanding why a peptide derived from neuroendocrine tissue would produce effects at the level of chromosomal architecture