Measurement of reactive oxygen species (ROS) and reduced glutathione (GSH) After maturation, the oocytes were incubated in 10 M CM-H 2 DCFDA (Thermo Fisher Scientific, Barcelona, Spain) and 10 M Cell Tracker Blue (Thermo Fisher Scientific, Barcelona, Spain) for 30 min at 37 C in the dark to detect intracellular reactive oxygen species (ROS) and reduced glutathione (GSH), respectively
GSTO1 C4-10 crystals had no additional cryoprotection
Jimnez-Castro MB, Cornide-Petronio ME, Gracia-Sancho J, Peralta C
The impurity content of reduced glutathione is as follows: impurity A Chemical name: bis (L-gamma-glutamyl-L-cysteinylglycine) disulfide (L-oxidized glutathione) Structural formula (xvi): the molecular formula is as follows: C20H32N6O12S2 Molecular weight: 612.63 Impurity B Chemical name: l-cysteinylglycine Structural formula (xvi): the molecular formula is as follows: C5H10N2O3S Molecular weight: 178.21 Impurity D Chemical name: (5S) -2-pyrrolidone-5-carboxylic acid Structural formula (xvi): the molecular formula is as follows: C5H7NO3 Molecular weight: 129.11 Compatibility test results In combination with domestic common solvents for injections, 0.9% sodium chloride injection, 5% glucose injection, 10% glucose injection and water for injection are selected for compatibility experiments under normal temperature conditions
B., Vernardis, S