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After 48 h, DMEM was replaced with Neurobasal medium (Gibco, 21103049) containing 50 B27 (Gibco, 17504044), 100 glutamine (Invitrogen, 25030081), and 100 penicillin-streptomycin (Thermo, 15140122)

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Further studies are required to fully elucidate the potential of the glucagon tracer presented here as a valuable tool for receptor target engagement and drug development