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pAAV-hSyn-DIO-ChrimsonR-mRuby2-ST, plasmid from Addgene, 105448, RRID: Addgene_105448-AAV9, virus packaged at UNC Vector Core (100 l at titre 1 10 13 vg ml 1 )

That part is going to be up to you, and this article was written to help you navigate these murky waters with ease

In addition, fatty acids other than palmitate can also be attached to the available Cys and might confer different binding specificities to the S-acylated protein 31