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"Glutathione synthesis." Biochim Biophys Acta

S-5-HT was measured with the HPLC-ECD chromatographic method ( st and the 2 nd samples were analyzed independently, as soon as possible after each blood draw, using the same method described above

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Cooled the mixture to 25-30C and stirred 10 hr at same temperature

Precision was calculated as the relative standard deviation, whereas accuracy was considered as the percentage of analyte recovery using the following formula: accuracy (%) = 100% x (measured amount - endogenous content)/added amount