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The tissue sections of plerocercoids and adult worms was first retrieved after microwaving for 20 min with a 0.01 M citric acid buffer (pH 6.0), blocking with 5% normal goat serum in PBS, then incubating at 37C or 1 h with a 1: 10 dilution of anti-rSmGST serum, serum of mice infected with plerocercoids, and normal mouse serum or PBS, respectively
Cousins RJ
This combination creates conditions where nausea may develop
Symptoms include heavy periods, breast tenderness, water retention, and mood changes
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