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n = 5 mice per experimental group
Specifically : HPLC Analysis: 99% purity via reversed-phase chromatography with C18 columns, acetonitrile/water gradients, and TFA ion-pairing
predicting phenobarbital level based on loading dose Level ~ 1.5 [ Dose in mg/kg ] For every 1 mg/kg of phenobarbital administered, the serum phenobarbital level will rise by roughly ~1.5 mg/L (e.g., 10 mg/kg will increase the phenobarbital level by 15 mg/L)
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Additionally, the cells showed elevated levels of ceramide at the plasma membrane surface
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