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Antibodies used were: anti-keratin 16 (sc-53255, Santa Cruz)

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Cultures were maintained in a humidified atmosphere of 5% CO 2 at 37C for 24 h and then incubated for the next 24-h period in DMEM-10% HS additionally containing recombinant HGF that was treated with peroxynitrite with or without trisulfide pre-treatment (see Figure 1d,f)

250 l of Lipofectamine mixture was added to the siRNA mixture