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Spiking/recovery tests were performed to determine the optimal dilution factor, particularly for the kits lacking manufacturer-provided serum dilution guidelines [human pro-BDNF DuoSet ELISA kit (#DY3175, R&D Systems) and high-sensitivity pro-BDNF human ELISA kit (#SK00752-09, Aviscera Bioscience)]

Broccoli Contains sulfur compounds that enhance glutathione production

There are strong pieces of evidence suggesting that ferroptosis exacerbates inflammation through immunogenicity, and several ferroptosis inhibitors have exhibited anti-inflammatory capabilities in various laboratory models of diseases (Kim et al., 2019)