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In this case, 5-h-fasted mice were intraperitoneally administered with 0.75 U of insulin (Sigma-Aldrich, MO, USA) per kg body weight, and blood glucose concentrations were determined before (0) and at 15, 30, 60, and 120 min after insulin injection
Plasma nonesterified fatty acid (NEFA) was measured using a NEFA-C assay kit (WAKO, Osaka, Japan)
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