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Despite these limitations, current guidance recommends a diet that leads to a caloric deficit

Animal experiments were carried out under approval by the Institutional Animal Experiment Committee of the Jichi Medical University, and in accordance with the Institutional Regulation for Animal Experiments and Fundamental Guideline for Proper Conduct of Animal Experiment and Related Activities in Academic Research Institutions under the jurisdiction of the Ministry of Education, Culture, Sports, Science and Technology

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Discontinuation of trials due to adverse events and other detrimental outcomes may lead to bias in the assessment of the final results