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Purified MDH (~1 mL) was dialyzed for 4 h in 1 L buffer 2 (20 mM MOPS-KOH pH 7.5, 150 mM NaCl) containing 10 mM EDTA followed by three times dialysis against 1 L of buffer 2

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American Journal of Clinical Nutrition, 106 , 888894

acute alcohol intake (such as during a binge) markedly increases both acetaldehyde production and oxidative stress

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