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Cells from a 50 mL overnight culture in LB medium were harvested by centrifugation (4,000 g, 10 min) and resuspended in 20 mL MOPS minimal medium ( 578 ~ 0.2) containing 50 g mL 1 ampicillin (pGEX-6P-1- cntAB - bcct ), 17 g mL 1 chloramphenicol (pACYCDuet-1- msadh - mdh ) and 500 M IPTG
Cell viability relative to untreated controls was quantified using a standard Cell Counting Kit-8 (CCK-8) assay (YEASEN, Shanghai, China)
Capryloyl Salicylic Acid (LHA) gently exfoliates without BHA irritation clearing the way for actives to absorb
Its used on demand rather than daily, so cumulative exposure is limited compared with maintenance medications
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