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qRTPCR was performed via SYBR Premix Ex Taq II (Takara) on a Chromo 4 continuous fluorescence detector with a PTC-200 DNA Engine Cycler (Bio-Rad Laboratories, Hercules, CA)
When water freezes, it forms ice crystals
[1] A frequently employed regimen involves applying approximately 1-2 mL of the 0.5 % solution to affected scalp regions once to twice daily, preferably on clean, dry skin in the evening
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