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estradiol (SMD = 2.722, 95% CI: 3.786 to 1.659, p = 0.0001)

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For each condition 1 mL supernatant was collected from the 150 cm 2 flask, centrifuged at 10,000 g and 4 C for 1 min, 500 L were transferred into a 1.5 mL collection tube, snap-frozen in liquid nitrogen, and stored at 80 C until analysis