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The coupling agent used in the reaction can be selected from group consisting of [Ethylcyano (hydroxyimino)acetate-02)-tri-(l-pyrrolidinyl)-Phosphonium hexa fluorophosphate (PyOxim), ethyl-2 -cyano-2-(hydroxy amino) acetate (Oxyma pure), 0-(benzotriazol-l-yl)-N,N,N,N'- tetramethyluronium tetrafluoroborate (TBTU), diisopropyl carbodiimide (DIC), 1,3- dicyclohexylcabodiimide (DCC) , 0-(7 -azabenzotriazol- 1 -yl)-N,N,N',N'-tetramethyluronium hexafluorophosphate (HATU), 1 -(dimethyl aminopropyl)-3-ethylcarbodiimide hydrochloride (EDC HC1), 0-(benzotriazol-l-yl)-l,l,3,3-tetra methyluronium hexafluorophosphate (HBTU), 1- Hydroxybenzotriazole (HOBt), Isopropyl chloro formate (IPCF), Benzotriazol-l-yl-oxy- tris(dimethyl-amino)-phosphonium hexa fluorophosphate (BOP), benzotriazole-1- yloxytri(pyrrolidino)phosphonium hexa fluoro phosphate (PyBOP), N,N-bis-(2-oxo-3- oxazolidinyl)phosphonic dichloride (BOP-C1), bromotri(pyrrolidino)phosphonium hexa fluoro phosphate (PyBrOP), 0-(6-Chloro-l-hydrocibenzotriazol-l-yl)-l,l,3,3-tetramethyl uranium tetra fluoroborate (TCTU), chlorotri (pyrrolidino)phosphonium hexafluorophosphate (PyClOP), Ethyl l,2-dihydro-2-ethoxyquinoline-carboxylate(EEDQ), isobutyl chloro formate (IBCF), 2- succinimido-l,l,3,3-tetramethyluronium tetrafluoroborate(TSTU), l-Cyano-2-ethoxy-2-oxo ethylidene aminooxy) dimethyl amino morpholino-carbeniumhexafluorophosphate (COMU), 2- (5-norbornen-2,3-dicarboximido)-l , 1 ,3,3-tetramethyluronium tetrafluoroborate (TNTU), propane phosphonic acid anhydride (PPAA), 3-(diethoxy phosphoryloxy)-l,2,3-benzotriazin- 4(3H)-one (DEPBT) or its mixture

Peer review Peer review information Communications Biology thanks Chao Mao and the other, anonymous, reviewer(s) for their contribution to the peer review of this work

Most individuals begin noticing weight loss within the first few weeks, with continued progress over several months

The oral format requires daily administration according to prescribing information