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Slides were mounted with Fluoromount-G (Southern Biotech, USA) and examined using a Leica TCS SP5 AOBS confocal laser scanning microscope (Leica Microsystems, Germany) equipped with HCX PL APO 63 oil (NA = 1.4) objectives

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Erring on the side of caution with periodic assessment provides peace of mind and early warning of any unexpected effects

Research evidences have showed that Chaihu Shugan San can increase the expression of 5-HT1A receptor, and thus promoting cell proliferation in dentate gyrus of hippocampus in epileptic rats with a depressive state, induced by pilocarpine and chronic mild stress (CMS), effectively improving some depressive symptoms [144]