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After blocking with blocking solution (0.2% casein, 0.3% Triton X-100, and 3% goat serum diluted in PBS) for 2 h at room temperature, anti-C3 (1:100 dilution) antibody-stained sections were reacted overnight at 4 C

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The medium was replaced, and after 8 h the cell supernatant was collected and co-incubated with RAW264.7 cells for 4 h

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Pregnancy can be a time of increased oxidative stress due to hormonal changes, metabolic demands, and the presence of the developing fetus