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In short, the samples were put in ice-cold PBS and centrifuged for 20 s at 100 g at room temperature followed by washing three times with 37 C PBS and by digestion in trypsin and DNase at 37 C for 10 min
Food and Drug Administration (FDA) labels alert users to a small number of rarer but more serious adverse events
After that, you may shift to once-a-week injections for one to two months, and then to monthly injections
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doi: 10.1073/pnas.1403438111 97 TanLGurbaniDWeisbergELHunterJCLiLJonesDSet al
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