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Each batch passes through Janoshik Analytical for HPLC purity quantification of each of the two component compounds and MS identity confirmation, with the minimum acceptance threshold set at 99 percent purity per component by HPLC
estradiol: I 2 = 84.30%, Tau 2 = 1.372, Q = 31.841, df = 5, p = 0.0001
It comes from the C-terminal region of human growth hormone (hGH), specifically residues 176 to 191
Wash unbound components again and add TMB substrate solution
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