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Lean mass plays a major role in resting metabolic rate, and resting metabolic rate strongly influences weight stability over time

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thaliana mutant for a mitochondrial -cyanoalanine synthase (CAS-C1) that is normally able to detoxify hydrogen cyanide

Then, 20 L of MTT solutions was added to each well followed by an incubation period of 3 hours, then the media were removed and 200 l of DMSO was added to dissolve the crystal and the absorbance was recorded at 570 nm (BioTek, USA) and cell viability was calculated as a percentage compared to cells treated with DMEM alone

Nat Rev Urol 18(7):387403