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Analysis of mitochondrial membrane potential (m) The m was assessed using the JC-10 Staining Dye Assay Kit (Biosharp, #BL726A, China), following the manufacturers protocol
The condition causes significant pain, nausea, and digestive problems, and recovery depends on identifying and treating the underlying cause
It stores a large portion of the water it consumes in Standley Lake, which is also a water source for Westminster and Northglenn
Ethical principles of the study complied with the European Directive 010/63/E, the Law on Amendments to the Animal Protection Act (Official Gazette 37/13), the Animal Protection Act (Official Gazette 135/06), the ordinance on the protection of animals used for scientific purposes (Official Gazette 55/13), Federation of European Laboratory Animal Science Associations (FELASA) recommendations, and the recommendations of the Ethics Committee of the School of Medicine, University of Zagreb
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