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Moreover, despite the similarity in structure, IGF-1 and insulin show different distribution in tissues, different internalization kinetics and different subcellular distribution of hormonal receptors
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10.4161/epi.24916 Epigenetics 168 SadiqS.BerndtT
After pre-conditioning, cells were washed and incubated in glucose-free DMEM medium containing 20 M 2-NBDG for 30 min at 37 C
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