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Whether an amino acid was classified as nutritionally essential or nonessential is only the matter of definition, and should not reflect the physiological needs of animals or humans for the nutrient (Hou and Wu 2017, 2018)

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AG-haESC culture and transfection AG-haESCs carrying deletions in the H19 -DMR and IG -DMR regions, as well as an Oct4-EGFP transgene (termed H19 DMR - IG DMR -AGH-OG3 or 408), were used to generate EFS-C9 and CAG-C9 cells for genetic screening

References Rodriguez PJ, Zhang V, Gratzl S et al