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In addition, EDTA plasma was obtained as follows: female mice ( n = 38) were anaesthetised with isoflurane, and blood was drawn from the inferior vena cava, pooled into 6 ml EDTA-coated tubes (VWR) and centrifuged (1650 g , 4C, 15 min)

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This can cause irregular menstrual cycles or temporarily absent periods, disrupted ovulation timing where you might ovulate earlier or later than expected, changes in estrogen, progesterone, and other reproductive hormones, and temporary changes in cervical mucus quality

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