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Metformin also enhances endothelial function, improves lipid metabolism, reduces the intima-media thickness of the carotid artery, and lowers the risk of cardiovascular diseases such as hypertension ( 3.2.3 Impact of metformin on pregnant patients with polycystic ovary syndrome and preeclampsia Research has shown that metformin can inhibit the placental secretion of soluble fms-like tyrosine kinase 1 and promote arterial dilation, which suggests metformin may be a potential preventive and therapeutic agent for preeclampsia

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Absorbance readings were acquired using a microplate reader (Bio-Rad) at a wavelength of 450 nm