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Calculate the required volume of solvent - Use this formula: Required solvent volume (in mL)*= Total amount of peptide (in mcg) / Desired concentration (in mcg/mL) For example, to make a 250mcg/mL solution from a 5,000mcg (5mg) peptide vial: 5,000mcg / 250 mcg/mL = 20mL Your peptide supplier, if in doubt
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