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The residues Ile10, His5, Leu13, and Leu17 were identified as gate-keeper residues near the phenol escape channels
Other common reasons for waking during the night include external factors such as noise, intrinsic factors such as pain or reflux or constipation, problems with sleep maintenance, and parasomnias
Overall mouse health was assessed by daily inspection for signs of discomfort, weight loss, or changes in behaviour, mobility, and feeding or drinking habits
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Small, simple structures resist thermal degradation better
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