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pneumoniae isolates from 42 US states for genomic analysis, in order to elucidate the genetic diversity, transmission dynamics, and emerging lineages driving ESBL dissemination in the community
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Most are studied predominantly in male research populations
The gel pieces were destained in a solution containing 50% ACN in 100 mL of 25 mM ammonium bicarbonate ((NH 4 )HCO 3 ) and digested with trypsin for overnight at 37 C in 25 mM ((NH 4 )HCO 3 )
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