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Sinha et al., 2018

Document storage and handling conditions in the laboratory record

DTNB Solution

Crosslinking mass spectrometry (XL-MS) The final concentration of 0.5 M DDD-Ube2e2-COP1-c-Jun was incubated with a final concentration of 0.1 mM DSBU crosslinker (Thermo Fisher, A35459) in the wash buffer (20 mM HEPES-NaOH pH 7.4, 200 mM NaCl, 2 mM MgCl 2 , 0.5 mM TCEP) for 40 min at 25 C and quenched with 10 mM Tris-HCl pH 8.0 for 10 min at 25 C

After the sclerosant is injected, compressing the vein by hand can help close it and prevent it from refilling, avoiding trapping too much blood which causes hyperpigmentation