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Standard protocols were followed for calibration of the chambers
The mutant with G2 mutations + mutations from all three clones was named G3, which was subsequently named LaccID (Supplementary Fig
Following fixation, L3 filets were washed in PTX (PBS and 0.02% Triton X-100) and blocked in PBT (PBS, 0.02% Triton X-100 and 1% NGS) on a nutator and incubated with primary antibodies for either 2 h at room temperature or overnight at 4 C with agitation
Reporting of prescribing and preparation errors were also higher for compounded medications, the paper found
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