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Long-term depression of NMDA receptor-mediated synaptic transmission is dependent on activation of metabotropic glutamate receptors and is altered to long-term potentiation by low intracellular calcium buffering
25 units = 0.25mL 0.6mg/mL = 0.15mg per injection (starter vial)
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The per-vial version of the same maths is on the 5 mg vial protocol and 10 mg vial protocol pages
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