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At 24 h post-transfection, the total RNA was isolated from LMH cells, and the relative mRNA abundance was quantified to assess transfection efficiency [27]

Ethics approval and consent to participate The medical ethics committee of the Maastricht University Medical Center+, the Netherlands approved the TearAD study (identifier: NL73600.068.20/METC 20033), and all participants provided written informed consent

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However, results depend on consistency, dosage, and lifestyle habits