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The prototypical member, aspirin , is one of the oldest analgesics, in use as an anti-inflammatory therapy long before the molecular mechanics of inflammation had been discovered

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The intake of pain-relieving drugs increased in the placebo group by 3.8 pills per patient per month and was reduced in the Pycnogenol group by 15 pills per patient per month at the end of the study

Serum was separated from the blood using centrifugation at 3,000 x g for 10 min