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In the Boyden chamber assay, TB4 stimulated keratinocyte migration 23-fold over medium alone after just 45 hours, with as little as 10 pg of TB4 sufficient to produce a potent stimulatory response
Keep the lyophilized vial in a freezer, away from light, until youre ready to use it
The 7500 Fast Real-Time PCR System from Applied Biosystems (Thermo Fisher Scientific) was used to run the reaction
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