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Decreased Inflammation : Adipose tissue (body fat) produces inflammatory cytokines that contribute to systemic inflammation

LEfSe is known to reduce false positives and can highlight biological consistency across subclasses, while the KruskalWallis test used alone can achieve a lower false-negative rate compared to LEfSe 38

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Side Effect 3: Allergic Reactions Though rare, allergic reactions require immediate medical attention

If your research hypothesis involves dose-response relationship testing, include at least three dose groups (low, medium, high) with vehicle control rather than escalating within a single group