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Eluted peptides were desalted using trap column (1 mm 15 mm, Acclaim PepMap300 C18, 5 m, Thermo Fisher Scientific) for 5 min at a flowrate of 200 L/min and 0.1% formic acid as mobile phase

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PUFAs have also been reported to possess anti-inflammatory effects and are able to enhance mitochondrial function, reduce ROS generation and endoplasmic reticulum stress (Lepretti et al., 2018)

This amount of weight loss is considered important in the treatment of obesity

A 0.60 mL draw may be unusually large for one subcutaneous site