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2.7 RNA extraction and quantitative real-time PCR (qRT-PCR) Total RNA was extracted from PIG1 and PIG3V cells using TRIzol reagent (#15596018, Invitrogen, USA), and its concentration and purity were assessed using a NanoDrop 2000 device (Thermo Fisher, USA)
Monitoring of glycation, oxidative stress and inflammation in relation to the occurrence of vascular complications in patients with type 2 diabetes mellitus
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