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For researchers using IGF-1 LR3 as a tool to study these pathways, the practical advantage is that sustained receptor activation enables longer time-course studies of pathway dynamics
For instance, in breast cancer cells, low iron levels are associated with a lower expression of EMT markers ( Ferroptosis-Based Treatment of Cancer Stem Cells Higher iron levels do not necessarily relate to ROS levels and ferroptosis, but it has been proven that CSCs are highly sensitive to ferroptosis due to increased expression levels of TFR1, and thus ferroptosis-based treatment and therapeutic interference of iron homeostasis can have a curing effect on cancer (Mai et al., 2017)
For a control, a plasmid containing a mCherry gene under constitutive expression was isolated
Plant Cell 35 , 42174237 (2023)
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