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Demonstrated that GHK-Cu at 10 nM concentration upregulated VEGF and eNOS mRNA in dermal fibroblasts

IGF-1 induces muscle hypertrophy through satellite cell activation and increased protein synthesis in differentiated myofibers (Adams & McCue, 1998)

Nardi G, Cipollaro M

The whole processes need to be carried out on ice, and the enzyme activity should be determined on the same day, to avoid repeated freeze-thaw of the homogenate solution