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Week 1: Dose: 50mcg Frequency: Once, on a night when you can sleep in the next morning Timing: 2-3 hours before intended sleep Purpose: Assess basic tolerance and response Week 2: Dose: 75-100mcg Frequency: 2-3 times during the week Timing: 1-2 hours before bed Purpose: Establish baseline effective dose During this assessment phase, track sleep quality subjectively and, if possible, with a wearable device that measures sleep stages

Wainwright MS et al

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In situ hybridization (ISH) studies have revealed that GLP-1R mRNA is also expressed in the human brain PVH, with its expression significantly reduced in T2DM patients compared to controls, potentially contributing to homeostatic imbalance [90]