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Isolation, islet number and insulin secretion from primary murine islets Pancreatic islets were isolated from WT or GRK2 +/ male mice by perfusion through the bile duct, as previously described [91]
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The monitoring of GSH/GSSG ratio and the quantification of GSSG in biological samples are essential for evaluating the redox and detoxification status of cells and tissues in relation to the protective role of glutathione against oxidative and free-radical-mediated cell injury
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