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Western blotting Cell lysates were collected in RIPA buffer (150 mM NaCl, 1.0% NP-40, 0.5% sodium deoxycholate, 0.1% SDS (sodium dodecyl sulphate)and Western blotting was performed as previously described 49
This additive glucose-lowering effect is therapeutically desirable for improving glycaemic control but increases the risk of hypoglycaemia, particularly with glipizide's non-glucose-dependent mechanism