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Kanani et al, 1999)

To prepare 10 mM HCl: Take 1 mL of standard concentrated HCl (12 M) Add to 1,199 mL of sterile water yields 10 mM HCl Alternatively, purchase pre-prepared sterile 10 mM HCl or use a validated dilution from a laboratory stock solution Alternative Solvent: 1% Acetic Acid in Sterile Water Some protocols use 1% acetic acid (glacial acetic acid diluted in sterile water) as the initial reconstitution vehicle

[9] On the skin, besides discoloration, MB can rarely provoke sweating, rash, or photosensitivity

Chronic Psychological Stress Stress is one of the most powerful and underappreciated triggers of mast cell activation