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In the standard 2D cell cultures, cells reveal a cobblestone shape, whereas in more advanced models with a dynamic flow, cells mimic the physiologic shape of vascular ECs in vivo, acquiring an elongated shape, due to shear stress [75]

Quantitative PCR assays were performed using 480 SYBR Green Master reagent mix (Roche Life Science, 4707516001, murine probes) or TaqMan assays (human ETC genes) on a real-time fluorescence qPCR instrument (LightCycler 480II, Roche Life Science)

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