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Reporting summary Further information on research design is available in the Nature Portfolio Reporting Summary linked to this article

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Ferner RE, Dear JW, Bateman DN

Calorie restriction-induced weight loss and exercise have differential effects on skeletal muscle mitochondria despite similar effects on insulin sensitivity

Cross-sectional studies have shown that plasma and tissue glutathione concentrations decrease by approximately 10-15% per decade after age 45, and this decline is accelerated in conditions associated with chronic oxidative stress, including type 2 diabetes, cardiovascular disease, neurodegenerative diseases, HIV/AIDS, chronic liver disease, and chronic obstructive pulmonary disease (COPD) [2][3][10][11]