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The expression of rHrGSTm1 was the highest in the supernatant of bacteria induced by 0.5 mM IPTG for 8 h at 37 C, and a single band of rHrGSTm1 was obtained by elution with 500 mm imidazole (Fig

Gliotoxin is also the only toxin that has been isolated from the sera of patients with invasive aspergillosis

The oxidation steps, utilizing glucose-6-phosphate (G6P) as the substrate, occur at the beginning of the pathway and are the reactions that generate NADPH

24 h later, culture media were replaced with 1 mL of serum-free DMEM containing 50 ng/mL of recombinant hTNF- (Peprotech) and cultured for a further 24 h before collecting cells and supernatants