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It is important to note that these metabolic adaptations are generally physiological responses to weight loss rather than pathological changes

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Quantification of free fatty acid (FFA) and total cholesterol The content of FFA and total cholesterol were quantified using the FFA content assay kit (Solarbio, BC0595) and the total cholesterol content assay kit (Solarbio, BC1985), respectively

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Rats treated with colonic acetic acid infusions exhibited low plasma GLP-1 levels and high serotonin levels in plasma and colonic tissues