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Cells were centrifuged at 400 x g for 5 min to pellet, the supernatant media removed and the pellet resuspended in 10 mL fresh L-15 media
Bacteriostatic water is required for multi-dose vials
Each batch is 0.2 m sterile-filtered to remove potential microbial contaminants, then filled into sterile glass vials under aseptic conditions
- 5:00 p.m
Data 18 , 16371755 (1989)
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