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Blots were incubated overnight at 4 C with HRP-conjugated anti-nitrotyrosine mouse mAb (1:2500 dilution in CanGetSignal solution 1,NKB-101, Toyobo, Osaka, Japan), followed by ECL-detection on a FUSION SOLO.7S.EDGE imaging system (Vilber Lourmat, Marne-la-Valle, France)
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Measurement of interleukin (IL)-1 and tumor necrosis factor- (TNF-) The inflammatory cytokines, TNF-, and IL-1 were quantified using ELISA kits (430915 and 432615
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